Description
The U.S. Environmental Protection Agency’s (USEPA’s) National Homeland Security Research Center (NHSRC) is currently working with laboratories and subject matter experts (SMEs) to optimize and verifyculture-based methods for S. Typhi and V. cholerae O1 and O139 in environmental matrices (e.g., drinking water,surface water). Once the culture component has been optimized, a polymerase chain reaction (PCR) protocol willbe optimized for use with the enrichment step included in each culture method; this will allow laboratories to utilizethe same upfront processing for either culture or molecular analyses. Most of the available culture-based protocolswere not developed or optimized for environmental matrices, requiring some method development work to addressbackground flora, complex environmental matrices (e.g., surface waters), and spiking procedures to evaluaterecoveries.
Product Details
- Edition:
- Vol. – No.
- Published:
- 11/01/2009
- Number of Pages:
- 3
- File Size:
- 1 file , 770 KB
- Note:
- This product is unavailable in Ukraine, Russia, Belarus